Antiprotozoal Nor-Triterpene Alkaloids from Buxus sempervirens L.

Malaria and human African trypanosomiasis (HAT; sleeping sickness) are life-threatening tropical diseases caused by protozoan parasites. Due to limited therapeutic options, there is a compelling need for new antiprotozoal agents. In previous study, O-tigloylcyclovirobuxeine-B was recovered from B. sempervirens L. (common box; Buxaceae) leaf extract bioactivity-guided isolation. This nor-cycloartane alkaloid identified as possessing strong selective in vitro activity against the causative agent of malaria tropica, Plasmodium falciparum (Pf). The purpose this study isolation additional alkaloids search further related compounds with activity. conclusion, 25 were obtained L., including eight natural products one compound first described plant. structure elucidation accomplished UHPLC/+ESI-QqTOF-MS/MS NMR spectroscopy. isolated tested Pf Trypanosoma brucei rhodesiense (Tbr), East sickness. To assess their selectivity, cytotoxicity mammalian cells (L6 cell line) well. Several displayed promising pathogens sub-micromolar range concurrent high selectivity indices (SI). Consequently, various have potential serve novel lead structure.